Amperometric Screen-Printed Galactose Biosensor for Cell Toxicity Applications

© 2016, Copyright © Taylor & Francis Group, LLC. ABSTRACT: This paper reports the development and application of a biosensor for the amperometric determination of galactose in the presence of human hepatocellular carcinoma cells with and without a hepatotoxic agent. The biosensor was fabricated by drop-coating 1.5% cellulose acetate on a 3×3mm screen-printed carbon electrode followed by depositing 2 U of galactose oxidase. The electrodes dimensions were reduced to 3×0.5mm before measurements. Hepatocellular carcinoma cells were utilized for in vitro toxicity testing by evaluating the effect of paracetamol on galactose uptake. The amperometric responses to galactose indicated that the inhibition of uptake was directly proportional to the concentration of paracetamol following 24h of exposure to the hepatocellular carcinoma cells. These results demonstrate that the fabricated biosensor may be used for the real-time monitoring of cell metabolism and toxicity

EU migrant retention and the temporalities of migrant staying:a new conceptual framework

Challenges of weak economic growth, population decline, and labour shortages led many countries across the world to introduce immigration policy changes in order to attract foreign migrants. This paper focuses on Japan (Tokyo) and the UK (Birmingham, Edinburgh and Glasgow) given common concerns over long term demographic trends and the burgeoning lack of labour supply in particular sectors of the economy through use of foreign labour. The paper shifts the focus from efforts focused on attracting and selecting foreign labour to the retention of such individuals. Drawing on research with EU migrants in Japan and the UK, the paper highlights how staying may occur after a period of mobility, rather than only being of relevance to those who never left their home region. The paper develops a new conceptual framework, which helps to identify different dimensions that shape migrant staying as a temporal process. It is highlighted how staying is shaped incrementally and facilitated or undermined over time in relation to the reciprocal importance of diverse assets, anchors and the changing biographies of migrants and the places in which they live – as well as the relational aspects of migrants’ ‘linked lives’

Are We All Agreed? Consensual Methods and the "Necessities of Life" in the UK Today

permits unrestricted re-use, distribution, and reproduction in any medium, provided the original work is properly cited. doi:10.1017/S004727941500003

Fabrication and evaluation of a micro(bio)sensor array chip for multiple parallel measurements of important cell biomarkers

© 2014 by the authors; licensee MDPI, Basel, Switzerland. This report describes the design and development of an integrated electrochemical cell culture monitoring system, based on enzyme-biosensors and chemical sensors, for monitoring indicators of mammalian cell metabolic status. MEMS technology was used to fabricate a microwell-format silicon platform including a thermometer, onto which chemical sensors (pH, O2) and screen-printed biosensors (glucose, lactate), were grafted/deposited. Microwells were formed over the fabricated sensors to give 5-well sensor strips which were interfaced with a multipotentiostat via a bespoke connector box interface. The operation of each sensor/biosensor type was examined individually, and examples of operating devices in five microwells in parallel, in either potentiometric (pH sensing) or amperometric (glucose biosensing) mode are shown. The performance characteristics of the sensors/biosensors indicate that the system could readily be applied to cell culture/toxicity studies

Experimental study of digital image processing techniques for LANDSAT data

The author has identified the following significant results. Results are reported for: (1) subscene registration, (2) full scene rectification and registration, (3) resampling techniques, (4) and ground control point (GCP) extraction. Subscenes (354 pixels x 234 lines) were registered to approximately 1/4 pixel accuracy and evaluated by change detection imagery for three cases: (1) bulk data registration, (2) precision correction of a reference subscene using GCP data, and (3) independently precision processed subscenes. Full scene rectification and registration results were evaluated by using a correlation technique to measure registration errors of 0.3 pixel rms thoughout the full scene. Resampling evaluations of nearest neighbor and TRW cubic convolution processed data included change detection imagery and feature classification. Resampled data were also evaluated for an MSS scene containing specular solar reflections